Applications · Organ perfusion

Keeping organs alive
is a fluid problem.

Machine perfusion keeps donor organs functioning outside the body by recirculating warm, oxygenated perfusate through them. As runs stretch from hours toward days, every part of the fluid path matters: how gently it moves the perfusate, how steadily it holds flow, and how long it stays closed. It is the same problem we solve every day for the most fragile biologics.

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INTERPHEX Exhibitor Awards 2026 winner, Best Technology InnovationINTERPHEX 2026 award winner, Best Technology Innovation

What does an organ perfusion system need from its fluid path?

Ex vivo machine perfusion pumps a perfusate, often blood-based, through an organ's vasculature at close to physiological temperature, flow and pressure, so the organ stays metabolically active and its function can be assessed. The fluid path has to stay closed and sterile for the whole run, move the perfusate gently enough to limit damage to blood cells, hold flow steady and precisely controllable, allow continuous additions of nutrients and medications, and record every reading so viability can be judged.

The challenge

Longer runs ask more
of the fluid path.

Closed and sterile, for longer

Clinical devices preserve organs for hours to about a day. Research is pushing toward several days.

Gentle on the perfusate

Hemolysis and platelet damage build up over a run, and the longer the run, the more they matter.

Steady, controllable flow

Liver perfusion alone runs two circuits, arterial and portal, at different flows.

Continuous additions

Nutrients, medications and other components are infused into the circuit throughout the run.

Every reading on record

Viability is judged from perfusate and flow data, so the run has to be monitored end to end.

What we provide

A reservoir, a pump and a system,
designed as one fluid path.

Dymaxion 2.5D single-use bag, front and side views, showing hanger, custom ports, sanitary clamp drain port and tapered base HangerCustom port layoutDrain portHanger sleeveTapered 2.5D base

Single-use perfusate reservoirs and bags

  • Drains to one point. The tapered 2.5D base guides perfusate to a single drain port that feeds the pump by gravity.
  • Ports where the circuit needs them. Return, sampling and addition ports, tubing and sterile connectors configured for each perfusion circuit.
  • USP Class VI film. Multi-layer film with an inert polyethylene contact layer, 13.5 mil (0.34 mm) thick.
  • Protected by five granted patents. Cut, welded and assembled in our own cleanroom, from first prototype to repeat supply.
More on our single-use bags ›
PIXER single-use diaphragm pump with inlet, outlet and injection lines

PIXER®: gentle, precise pumping for perfusion

  • No tubing squeezed. A diaphragm pump, not a roller pump: the perfusate is never crushed between rollers, and nothing wears or sheds into the circuit.
  • Gravity-fed inlet. Perfusate arrives under positive pressure, so the pump never pulls and there is no suction-side cavitation.
  • Steady, precise flow. An odd number of diaphragms keeps flow steady, from under 1 mL/min to liters per minute across the range, with turndown up to 3,000:1.
  • Metered additions. Injection ports add nutrients and medications directly into the flow, at the ratio you set.
More on PIXER® ›
Complete circuitsReservoirs, PIXER® pumps, routing valves, sensors and control designed together as one closed, single-use fluid path, with real-time data on how the run is performing. The bag's drain port feeds the pump inlet by gravity, so the perfusate is never pulled.

The control layer · Powered by ConSynSys

Hours or days on the machine.
Every minute on record.

ProCaaSo®, the cloud-native process control platform from our sister company ConSynSys, runs the perfusion circuit as one coordinated system and turns everything that happens into data the team can see, trust and use.

Sub-second

Closed-loop control

Pump speed adjusted continuously from live sensor data, with a control loop designed to react in under a second.

Full walk-away automation

Recirculation and additions run as recipes, start to finish, with alerts when the team is needed.

Viability data, in context

Every flow, pressure, alarm and intervention is recorded, with each reading tied to the step that produced it.

Bank-grade security

Zero-trust, encrypted, single-tenant cloud. Control runs locally and carries on if the network drops.

Explore ProCaaSo® Visit ConSynSys ›

ProCaaSo® is developed by our sister company, ConSynSys Technologies.

How we work with you

From your protocol
to a working circuit.

01

Understand the protocol

Organ, circuits, flows, temperature, run length and additions.

02

Prototype

Custom reservoirs and a circuit sized to your organ and model.

03

Iterate

Fast design turns as your program learns.

04

Scale

One architecture from research rigs to larger programs.

Why Alphinity

Built for the most
fragile fluids we know.

Our equipment was designed for viral vectors, mRNA and cell therapies: products that lose potency when they are pumped too hard, squeezed through tubing or exposed to cavitation. The same principles, a flooded inlet, steady flow, no crushed tubing and a closed single-use path, are what a perfusate needs over a long run.

Our TFFi™ system, built on the same pump and fluid path, won Best Technology Innovation at INTERPHEX 2026.

Technician in cleanroom gown, hairnet, beard cover and gloves assembling a single-use bag by hand
Every bag is cut, welded and assembled by hand in our own cleanroom.

FAQ

Common questions

What is machine perfusion of organs?

Machine perfusion keeps a donor organ functioning outside the body by pumping a preservation solution, called perfusate, through its blood vessels. Unlike static cold storage on ice, the organ is supplied with oxygen and nutrients, which allows its function to be maintained and assessed before transplantation.

What is normothermic machine perfusion?

Normothermic machine perfusion recirculates warm, oxygenated perfusate, often blood-based, through an organ at close to body temperature. The organ stays metabolically active, so clinicians and researchers can monitor its function during the run. Liver perfusion, for example, typically uses two circuits, with arterial flows around 0.2 to 0.3 L/min and portal flows around 0.8 to 1.25 L/min.

How long can an organ be perfused outside the body?

Clinical machine perfusion devices typically preserve organs for several hours up to about a day. In research, longer runs are being explored: published studies have perfused human kidneys for up to four days at subnormothermic temperature, and preclinical work has extended preservation toward a week.

Why does pump choice matter in organ perfusion?

The pump moves every drop of perfusate through the circuit, continuously, for the whole run. Pump design can affect hemolysis, platelets and inflammatory markers, and hemolysis becomes more of a problem the longer the run lasts. A gentle, low-shear pump that does not squeeze tubing, with steady and precisely controllable flow, reduces stress on the perfusate.

Can Alphinity supply components for organ perfusion research?

Yes. Alphinity designs custom single-use reservoirs and bags in its own cleanroom, on bag technology protected by five granted patents, together with PIXER low-shear diaphragm pumps and closed-loop control from sister company ConSynSys. Equipment is available to research and development programs. It is not approved for clinical use, and Alphinity works with partners on the regulatory pathway for clinical applications.

Related: Single-use bags · Artificial womb and ex utero fluid systems · PIXER® pumps

Published research: Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view. PMC10602690

A cell-free nutrient-supplemented perfusate allows four-day ex vivo metabolic preservation of human kidneys. Nature Communications, 2024. nature.com

Effect of centrifugal and roller pumps on blood and myocardial structure in a normothermic machine-perfused ex vivo porcine heart model. Scandinavian Cardiovascular Journal, 2025. tandfonline.com

Get Started

Building an organ
perfusion program?

Tell us about the organ, the circuits, the flows and how long the run has to last. We will help design its fluid path.

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For research and development programs. Not approved for clinical use. We work with partners on the regulatory pathway for clinical applications.

Ready to talk?

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